崅抦戝妛堛妛晹堚揱巕婡擻夝愅妛嫵幒
僩僢僾儁乕僕尋媶/嫵堢撪梕徯夘僗僞僢僼嬈愌
尋媶&嫵堢撪梕徯夘

仏尋媶徯夘


DNA傪拻宆偲偟偨mRNA偺崌惉斀墳乮揮幨乯偼丄RNA億儕儊儔乕僛II乮Pol II乯偲偄偆峺慺偵傛偭偰怗攠偝傟傑偡偑丄偙偺斀墳偼奐巒丄怢挿丄廔寢偺3偮偺僗僥僢僾偐傜惉傝傑偡丅揮幨偺奐巒斀墳偑堚揱巕敪尰偵偍偗傞庡梫側惂屼抜奒偱偁傞偙偲偼埲慜偐傜抦傜傟偰偄傑偟偨偑丄嵟嬤偙傟偵懕偔怢挿抜奒傕丄斀墳偑搑拞偱旕忢偵掆巭偟堈偄乮幚偵僎僲儉忋偺1/3偺堚揱巕偱揮幨奐巒捈屻偵Pol II偺掆巭偑婲偙偭偰偄傞乯偙偲丄嫄戝側堚揱巕乮椺偊偽Dystrophin乯偱偼慡挿偺揮幨偵堦擔嬤偔傕偺帪娫偑偐偐傞偙偲側偳偐傜丄廳梫側惂屼偺応偱偁傞偙偲偑擣幆偝傟偰偒傑偟偨丅偙傟傑偱偵丄偙偺抜奒傪惓傑偨偼晧偵惂屼偡傞揮幨怢挿場巕偑10屄埲忋扨棧偝傟偰偄傑偡偑丄偦偺偆偪偺偄偔偮偐偼娻傪偼偠傔偲偡傞昦婥偺敪徢偲枾愙偵娭楢偡傞偙偲偑柧傜偐偲側傝丄揮幨怢挿場巕昦偲偄偆幘姵奣擮偑嶻傑傟偰偄傑偡丅
丂 巹払傕偙傟傑偱偵TFIIF丄Elongin A側偳丄係偮偺揮幨怢挿場巕傪扨棧偟偰夝愅傪峴側偭偰偒傑偟偨 (Nature 1992, Science 1995, JBC 2000, 2002)丅嵟嬤Elongin A偺儂儌寚幐儅僂僗傪嶌惢偟傑偟偨偑丄Elongin A-/-戀巈桼棃偺嵶朎偱偼傾億僩乕僔僗槾恑傗憗婜嵶朎榁壔偑擣傔傜傟丄Elongin A偑僗僩儗僗懴惈偵娭梌偡傞偙偲偑柧傜偐偲側傝傑偟偨乮Cell Death Differ. 2007乯丅傑偨丄Elongin A偑Elongin BC偵壛偊偰Cul5丄Rbx2偲傕寢崌偟偰暋崌懱傪宍惉偟丄Pol II傪昗揑偲偡傞儐價僉僠儞儕僈乕僛(E3)偲偟偰傕婡擻偡傞偙偲傪敪尒偟傑偟偨乮EMBO J. 2008, F1000 Biology乯丅偝傜偵嵟嬤丄Elongin A偑恄宱掔嵶朎偐傜偺姶妎恄宱傊偺暘壔傪巜椷偡傞揮幨惂屼場巕偺敪尰墳摎偵娭梌偡傞偙偲偑柧傜偐偲側傝丄擼丄愐悜偵偍偗傞姶妎恄宱宯偺宍惉偵傕廳梫側栶妱傪墘偠偰偄傞偙偲偑敾柧偟傑偟偨乮Cell Reports 2012乯丅

仸壓婰偺僗儔僀僪偼僋儕僢僋偡傞偙偲偱戝偒側夋憸偱尒傞偙偲偑偱偒傑偡丅


僗儔僀僪1 僗儔僀僪2
僗儔僀僪3 僗儔僀僪3
僗儔僀僪3 僗儔僀僪3
僗儔僀僪3 僗儔僀僪3



仏嫵堢撪梕


仏妛晹嫵堢仏

亂堚揱巕堛妛亃

侾乯僎僲儉傪妛傇
僎僲儉,僩儔儞僗僋儕僾僩乕儉,僾儘僥僆乕儉丄DNA尋媶朄丄僎僲儉抧恾偺嶌惉丄僎僲儉攝楍偺夝愅丄僎僲儉攝楍傪棟夝偡傞丄僎僲儉偑偳偺傛偆偵偟偰婡擻偡傞偐傪棟夝偡傞

俀乯僎僲儉夝朥妛
恀妀惗暔僎僲儉丄尨妀惗暔僎僲儉偲恀妀惗暔偺嵶朎彫婍姱僎僲儉丄僂僀儖僗僎僲儉偲摦偔堚揱巕

俁乯僎僲儉偺婡擻
僎僲儉傊偺愙嬤丄揮幨奐巒暋崌懱偺宍惉丄RNA偺崌惉偲僾儘僙僔儞僌丄僾儘僥僆乕儉偺崌惉偲僾儘僙僔儞僌丄僎僲儉婡擻偺挷愡

係乯僎僲儉偺暋惢偲恑壔
僎僲儉暋惢丄曄堎偲DNA廋暅丄慻姺偊丄僎僲儉偺恑壔


亂堚揱巕堛妛幚廗亃
乮侾乯 儅僂僗娞憻偐傜偺愼怓懱DNA偲僞儞僷僋幙偺挷惢
乮俀乯 僞儞僷僋幙偺掕検偲SDS億儕傾僋儕儖傾儈僪僎儖揹婥塲摦
乮俁乯 DNA偺惂尷峺慺偵傛傞愗抐偲傾僈儘乕僗僎儖揹婥塲摦乮RFLP夝愅乯
乮係乯 PCR朄偵傛傞堚揱巕宆偺敾掕
乮俆乯 僾儔僗儈僪DNA偵傛傞戝挵嬠偺宍幙揮姺

仏戝妛堾嫵堢仏

亂堛壢妛愱峌廋巑壽掱亃
丂暘巕偐傜尒偨堛妛偲堛椕

亂堛妛宯尋媶壢攷巑壽掱亃
丂揮幨惂屼婡峔偲敪娻丄婎慴暘巕惗暔妛幚尡朄

乹儁乕僕偺僩僢僾傊乺


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