丂僗僼傿儞僑摐帀幙乮GSL乯偼丄嵶朎枌忋偱屳偄偵cis憡屳嶌梡偡傞偙偲偱夛崌偡傞惈幙偑偁傝丄帀幙儅僀僋儘僪儊僀儞乮帀幙儔僼僩偲傕屇偽傟傞乯傪嵶朎枌忋偵宍惉偡傞丅傑偨丄朞榓側傾儖僉儖嵔偱峔惉偝傟偰偄傞僌儕僙儘摐帀幙傗GPI傾儞僇乕宆僞儞僷僋幙傕帀幙峔憿偺摿挜偐傜帀幙儅僀僋儘僪儊僀儞傪嵶朎枌忋偵宍惉偡傞丅偙傟傜偺帀幙儅僀僋儘僪儊僀儞偼丄Src family kinase偺傛偆側枌偵夛崌偡傞嵶朎撪忣曬揱払暘巕傗丄惉挿場巕庴梕懱丒僀儞僥僌儕儞暘巕摍偑僪儊僀儞偵棧崌廤嶶偡傞偙偲偱丄惗暔婡擻偺敪尰傗挷愡偵娭梌偡傞偙偲偑帵嵈偝傟偰偄傞丅偟偐偟側偑傜丄偙傟傜偺帀幙儅僀僋儘僪儊僀儞傪夘偟偨嵶朎婡擻偺敪尰丒挷愡婡峔偺徻嵶偼枹偩偵晄柧側揰偑懡偄丅僸僩岲拞媴偺嵶朎枌忋偵偼Src family kinase偱偁傞Lyn偲夛崌偟偨儔僋僩僔儖僙儔儈僪乮LacCer乯偺枌儅僀僋儘僪儊僀儞偑偁傝丄岲拞媴偺梀憱丒婷怘丒妶惈巁慺嶻惗傪拠夘偡傞丅偙偺忣曬揱払偵偼丄扽慺悢24乮C24乯偺嬌挿嵔帀朾巁嵔傪傕偮C24-LacCer偑Lyn偲夛崌偡傞偙偲偑昁恵偲側偭偰偄傞丅堦曽偱丄僌儕僙儘摐帀幙偱偁傞儂僗僼傽僠僕儖僌儖僐僔僪(PtdGlc)傕岲拞媴偵摿堎揑偵敪尰偟丄LacCer偲偼堎側傞枌儅僀僋儘僪儊僀儞傪嵶朎枌忋偵宍惉偟丄Fas暘巕偲夛崌偡傞偙偲偱岲拞媴偵傾億僩乕僔僗傪桿摫偡傞丅偙傟傜偺偙偲偼丄嵶朎偵偼摐嵔偲帀幙偺僾儘僌儔儉偝傟偨敪尰惂屼婡峔偑懚嵼偟丄摐嵔傗帀朾巁嵔偺峔憿偺堘偄偵婎偯偄偨堎側傞婡擻僪儊僀儞偑摨堦偺嵶朎枌忋偵宍惉偝傟偰偄傞偙偲傪帵偟偰偄傞丅
丂偙偺僙儈僫乕偱偼丄摐帀幙偺惈幙偺堘偄偑枌儅僀僋儘僪儊僀儞偺峔憿偲婡擻偵偳偺傛偆偵娭傢偭偰偄傞偐偵偮偄偰偙傟傑偱偵柧傜偐偲側偭偰偄傞偙偲偵偮偄偰徯夘偟偨偄丅

丂僸僩偑戝杻乮儅儕僼傽僫乯傪愛庢偡傞偲丄摡悓姶丄帪娫丒嬻娫姶妎偺憆幐丄尪妎丄柊婥丄岥妷側偳偺徢忬偑惗偠傞偑丄偦傟偼戝杻偵娷傑傟傞僇儞僫價僲僀僪偑擼側偳偱G抈敀幙嫟栶宆偺僇儞僫價僲僀僪庴梕懱偲寢崌偡傞偐傜偱偁傞丅偙偺庴梕懱偺撪嵼惈儕僈儞僪偼乽僄儞僪僇儞僫價僲僀僪乿偲屇偽傟丄偦偺杮懱偼傾儔僉僪僲僀儖僄僞僲乕儖傾儈僪乮傾僫儞僟儈僪乯傗2-傾儔僉僪僲僀儖僌儕僙儘乕儖乮2-AG乯偺傛偆側傾儔僉僪儞巁桿摫懱偱偁傝丄惗懱枌偺儕儞帀幙偐傜峺慺揑偵嶌傜傟傞丅偙偺偆偪2-AG偼丄僔僫僾僗偺媡峴惈僔僌僫儖暘巕偲偟偰廳梫側惗棟嶌梡傪扴偭偰偄傞偙偲偑柧傜偐偵側偭偰偄傞丅堦曽丄傾僫儞僟儈僪偲椶帡偺峔憿傪帩偮僷儖儈僠儞巁傗僆儗僀儞巁偺僄僞僲乕儖傾儈僪傕懱撪偵懚嵼偟丄僇儞僫價僲僀僪庴梕懱偲偼寢崌偟側偄偑丄妀撪庴梕懱PPAR兛側偳偲寢崌偟丄峈墛徢丒捔捝丒怘梸梷惂側偳偺嶌梡傪帵偡丅庬乆偺帀朾巁偺僄僞僲乕儖傾儈僪偼嫟捠偺戙幱宱楬偱惗惉偡傞偲峫偊傜傟丄旝検偟偐懚嵼偟側偄傾僫儞僟儈僪偼丄偙偺宱楬偺暃嶻暔偺壜擻惈偑偁傞丅杮僙儈僫乕偱偼僄儞僪僇儞僫價僲僀僪偺嶌梡傪夝愢偡傞偲偲傕偵丄帀朾巁僄僞僲乕儖傾儈僪偺戙幱偵娭偡傞変乆偺峺慺妛揑抦尒傪徯夘偡傞丅

丂嵶朎偲恊偟偄恖岺峔憿懱偺儌僲偯偔傝傪捠偟偰丄嵶朎偺惗妶娐嫬偲戙幱惂屼偺巇慻傒偺戝愗偝丄恖娫偺埨怱埨慡偲惗妶娐嫬丄帺慠偲恖娫惗妶偲偺惍旛丄偦傟傜偵憡墳偟偄僀僲儀乕僔儑儞傪崅偄怣棅惈偑梫媮偝傟傞怴嶻嬈偵揔梡丄傑偨丄挻旝彫側媄弍偑戝偒側僀僲儀乕僔儑儞傊偲楢側偭偰擾妛丒岺妛丒堛妛丒栻妛側偳偺愱栧媄弍丒擾岺堛栻楢実偑恖娫傪巒傔惗暔偵桪偟偄幮夛娐嫬傊懳墳偟偰偄傞偙偲偵偮偄偰徯夘偟丄嵟屻偵丄崅抦戝妛偺庒偄尋媶幰傗妛惗彅孨傊偺儊僢僙乕僕偵偰丄儌僲偯偔傝傊偺巚偄傪揱偊偨偄丅
丂杮尋媶偺婲尮偼丄偑傫壔妛椕朄偵幏擮傪擱傗偟偨庒偄寣挭偑乽懴惈壔偲暃嶌梡偺嫹娫乿偺帯椕尷奅偵撍偒摉偨傝丄姵幰偲嫟偵怺偔擸傒挿偔嬯偟傒丄堛妛尋媶偵恖惗傪曭偘偨帺暘偺柌傪橰傒夁偛偟偰偄偨帪偵丄傆偲惗傑傟偨乽嵶朎偲恊偟偄拠娫憂傝乿偺婏憐揤奜側敪憐偱偁偭偨丅
丂崅抦戝妛偲丄尰嵼丄乽梊杊擾朄偺妋棫乿偵岦偗扵嶕傪恑傔丄崱屻丄峔憿懱婎斦偺嫮壔丄怘暔傾儗儖僊乕偺梊杊偲帯椕丄擄帯惈姶愼徢梊杊懳嶔丄偑傫揮堏梊杊堛妛偵丄柌偁傞尋媶幰偲偺暆峀偄楢実傪傕偲偵丄悽奅傪曄偊傞乽偄偛偭偦偆乿拠娫傪曞傝偨偄丅

丂僄僗僩儘僎儞偵恄宱曐岇偺摥偒偑偁傞偙偲偑拲栚偝傟偰偒偰偄傞丅摿偵丄彈惈偵懡敪偡傞懡敪惈峝壔徢傊偺椪彴墳梡偵僄僗僩儘僎儞偑帋峴偝傟偰偄傞丅拞悤恄宱宯偵偍偄偰丄桳悜恄宱偺惗懚堐帩偼儈僄儕儞宍惉嵶朎僆儕僑僨儞僪儘僒僀僩(OL嵶朎)偵埶懚偟偰偍傝丄 恦懍側儈僄儕儞嵞宍惉偼丄扙悜傪敽偆恄宱曄惈偐傜幉嶕傪曐岇偡傞丅偙偺傛偆側廳梫側栶妱偑帵偝傟偰偄傞偵傕娭傢傜偢丄OL嵶朎偺恄宱曐岇偺儊僇僯僘儉丄偲傝傢偗丄僄僗僩儘僎儞嶌梡偺暘巕儊僇僯僘儉偼晄柧偱偁傞丅嵟嬤丄G僞儞僷僋幙嫟栶庴梕懱GPR30偑枌宆僄僗僩儘僎儞庴梕懱偱偁傞偲曬崘偝傟偨丅杮尋媶偱偼丄GPR30偵徟揰傪偍偒丄OL嵶朎偱偺嬊嵼丄扙悜曄惈娚榓嶌梡傊偺塭嬁傪夝愅偟偨丅柶塽慻怐妛揑夝愅偵傛傝丄儔僢僩擼椑偍傛傃愐悜偺敀幙偺OL嵶朎偑丄GPR30傪敪尰偟偰偄傞偙偲偑柧傜偐偲側偭偨丅傑偨丄擼桼棃丄愐悜偺OL嵶朎弶戙攟梴偵偍偄偰丄GPR30偑OL嵶朎慜嬱嵶朎偐傜OL惉弉夁掱傪捠偟偰嵶朎懱偵嫮偔敪尰偟偰偄偨丅偦偺惗棟婡擻傪柧傜偐偵偡傞偨傔偵丄僋僾儕僝儞搳梌偵傛傞扙悜-嵞儈僄儕儞壔儌僨儖儔僢僩傪嶌惉偟丄GPR30摿堎揑傾僑僯僗僩G1偺搳梌偑儈僄儕儞宍惉偵梌偊傞塭嬁傪夝愅偟偨丅偦偺寢壥丄G1搳梌孮偺擼椑偵偍偄偰丄OL惉弉偑懀恑偟偰偄偨丅傑偨丄屻崻恄宱愡偲OL慜嬱嵶朎偺嫟攟梴宯偵偍偄偰丄G1偼丄儈僄儕儞宍惉懀恑嶌梡傪帵偟偨丅僄僗僩儘僎儞偑GPR30傪夘偟丄OL嵶朎偺惉弉桿摫丒儈僄儕儞宍惉偵摥偒丄恄宱曐岇偵岠壥揑側栶妱傪壥偨偟偰偄傞偲峫偊傜傟傞丅

丂師悽戙僔乕働儞僒乕偼丄偦偺搊応偐傜7擭偑宱夁偟丄暆峀偄尋媶幰偵擣抦偝傟傞夝愅憰抲偺堦偮偲側傝傑偟偨丅$1,000僎僲儉峔憐偺壓丄暷崙傪拞怱偲偟偰旕忢偵懡偔偺儀儞僠儍乕婇嬈傗尋媶慻怐偑怴偟偄僔乕働儞僔儞僌媄弍偺奐敪嫞憟傪孞傝峀偘偰偍傝傑偡丅偦偺傛偆側拞丄2011擭4寧27擔偵暷崙Pacific Biosciences幮偑廬棃偺師悽戙僔乕働儞僒乕偲偼慡偔堎側傞怴偟偄媄弍傪梡偄偨戞嶰悽戙僔乕働儞僒乕PacBio RS傪敪昞偟傑偟偨丅2011擭枛偐傜偼擔杮偱偺摫擖傕奐巒偝傟丄廬棃偺師悽戙僔乕働儞僒乕偲嫟懚偟側偑傜妶梡偝傟傞偙偲偑婜懸偝傟偰偄傑偡丅
丂杮僙儈僫乕偱偼傑偢巒傔偵丄PacBio RS偺尨棟偵偮偄偰偛愢柧偟傑偡丅PacBio RS僔乕働儞僒乕偱偼丄廬棃偺師悽戙僔乕働儞僒乕偲偼堎側傝丄(1) 1暘巕儕傾儖僞僀儉僔乕働儞僔儞僌偵傛傞DNA崌惉偲専弌偺摨帪幚峴丄(2) 偙傟傑偱偺婰榐傪偼傞偐偵忋夞傞乬挻儘儞僌儕乕僪乭偺幚尰丄(3) PCR偵傛傞憹暆夁掱偑晄梫丄(4) GC娷桳検偵娭傢傜側偄嬒堦側僔乕働儞僗寢壥偺庢摼丄(5) DNA偺儊僠儖壔偲偄偭偨僄僺僕僃僱僥傿僋僗尋媶偵偍偗傞峀斈側墫婎廋忺偺専弌丄傪幚尰偟傑偟偨丅傑偨丄PacBio RS偵偍偗傞儚乕僋僼儘乕偍傛傃幚嵺偺墳梡椺偵偮偄偰傕偛徯夘偝偣偰偄偨偩偒傑偡丅偙傟傪婡夛偵丄PacBio RS僔乕働儞僒乕偺偛棙梡傪偛専摙捀偔偲嫟偵丄奆條偺偛尋媶偺敪揥偵峷專偱偒傑偟偨傜岾恟偱偡丅

丂My studies presently aim to answer : What are the structural proteins of the mitotic chromosome and how do they direct chromosome segregation in mitosis? Currently, we have identified ~4,000 polypeptides in highly purified chicken mitotic chromosomes by using a mass-spectrometry based proteomic analysis. This seminar is going to focus on a novel approach that we term Multi-Classifier Combinatorial Proteomics, to sort through this massive proteomic data set and integrate multiple classifiers by machine learning uncovered functional relationships between protein complexes in the context of intact chromosomes.

丂偙偺偨傃丄崅抦戝妛堛妛晹嵟弶偺僥僯儏傾丒僩儔僢僋嫵堳偲偟偰丄愭抂堛椕妛悇恑僙儞僞乕偵懢揷怣嵠愭惗偑拝擟偄偨偟傑偟偨丅丂懢揷愭惗偺偙傟傑偱偺尋媶嬈愌偲崱屻偺書晧偵偮偄偰徯夘偟偰偄偨偩偒傑偡丅

丂儔僼僩偼僗僼傿儞僑帀幙偲僐儗僗僥儘乕儖偵晉傓嵶朎枌偺儈僋儘僪儊僀儞偱偁傞丅條乆側婡擻僞儞僷僋幙傪廤愊偝偣傞偙偲偵傛傝丄枌傪夘偡傞僔僌僫儖揱払傗嵶朎愙拝偺拞宲揰偲偟偰摥偄偰偄傞丅 寣娗偑彎偮偔偲寣塼嬅屌斀墳偑奐巒偝傟丄僼傿僽儕僲乕僎儞偑僩儘儞價儞偵傛傞愗抐偱僼傿僽儕儞偵曄傢傝僼傿僽儕儞偺栐偑寣愷偲偟偰彎岥傪屌傔傞丅傑偨丄寣塼偑嬅屌偟偨偺偪偵僼傿僽儕儞栐偲寣彫斅偐傜側傞嬅寣夠偑廂弅偡傞寣栞戅弅偲偄偆尰徾偑抦傜傟丄宍惉偟偨寣愷傪嫮屌偵偟偰巭寣傪姰慡偵偡傞摥偒傪偟偰偄傞丅寣彫斅枌偺僼傿僽儕儞庴梕懱GPIIb/IIIa(僀儞僥僌儕儞兛IIb兝3)偺嵶朎幙懁偵儈僆僔儞偑寢崌偟丄傾僋僩儈僆僔儞宯偺廂弅偵傛傝僼傿僽儕儞慄堐傪堷偭挘傞偙偲偱偍偙傞偲峫偊傜傟偰偄傞丅巹偨偪偼寣彫斅傪僩儘儞價儞偵傛傝妶惈壔偡傞偲丄僼傿僽儕儞偍傛傃儈僆僔儞偑儔僼僩夋暘偵堏峴偡傞偙偲傪尒弌偟偨丅傑偨寣彫斅傪柶塽愼怓偡傞偲丄寣彫斅枌偺慡懱偱偼側偔尷嬊偟偨椞堟偵僼傿僽儕儞丄妶惈壔儈僆僔儞丄僗僼傿儞僑帀幙偑嫟嬊嵼偡傞偙偲偑傢偐偭偨丅偙偺偙偲偐傜丄奜懁偺僼傿僽儕儞慄堐偲撪懁偺傾僋僩儈僆僔儞宯偑寣彫斅枌偺儔僼僩傪夘偟偰岠棪傛偔楢寢偟丄寣栞戅弅偵昁梫側挘椡傪惗傒弌偟偰偄傞壜擻惈偑峫偊傜傟偨丅丂 丂丂丂丂

丂儕僺僪儈僋僗傪偼偠傔偲偡傞儊僞儃儘乕儉夝愅偺摿挜偼丄惗棟揑丄昦棟揑娐嫬側偳偺堎側偭偨宯偵偍偗傞懡悢偺峔惉暘巕傪曪妵揑偵暘愅偟丄偦偺僾儘僼傽僀儖傪斾妑偡傞偙偲偵傛傝丄嵟傕偦偺尰徾偲娭楢偡傞壜擻惈偺崅偄場巕孮傪扵傝弌偡偲偄偆揰偵偁傞丅尰嵼変乆偑尋媶懳徾偲偟偰偄傞帀幙偼僜僼僩僀僆儞壔幙検暘愅偺敪揥偵傛傝丄偦偺徻嵶側暘巕庬偺懚嵼偑柧傜偐偵偝傟丄偦傟傜偑惗棟揑昦棟揑偵堎側傞栶妱傪扴偭偰偄傞偙偲偑敾偭偰偒偨丅変乆偼帀幙儊僞儃儘儈僋僗夝愅傪婎斦偵丄帀幙戙幱堎忢徢丄墛徢側偳傪儌僨儖偲偟偰丄帀幙戙幱暘巕偺僾儘僼傽僀儖偺曄摦偺曪妵揑側夝愅丄摿偵崅搙晄朞榓帀朾巁娷桳帀幙偲偦偺巁壔暔偺婡擻偲昦懺傊偺娭梌偺夝柧摍傪庡偨傞僥乕儅偲偟偰尋媶傪恑傔偰偒偨丅 丂
丂偙偺僙儈僫乕偱偼丄儕僺僪儈僋僗偱梡偄傜傟傞庡梫側夝愅庤朄偵偮偄偰夝愢偟丄変乆偺尋媶傪椺偵丄惗柦壢妛尋媶傗憂栻奐敪摍偵儕僺僪儈僋僗庤朄傪揔梡偡傞嵺偺峫偊曽摍偵偮偄偰夝愢偡傞丅傑偨丄嵟嬤丄変乆偑庢傝慻傫偱偄傞梟攠拪弌昞柺暘愅朄偲偄偆慻怐嬊強偵偍偗傞帀幙暘巕庬偺捈愙夝愅朄偵偮偄偰傕怗傟偨偄丅 丂丂丂丂

丂恄宱宯傗柶塽宯側偳偺崅師婡擻僔僗僥儉傪峔抸偟丄偦偺暋嶨側婡擻傪敪尰丒堐帩偟偰偄偔偨傔偵偼丄奺乆偺宯傪峔惉偡傞嵶朎孮偺僟僀僫儈僢僋側宍懺曄壔偑昁梫偲側偭偰偔傞丅偙偺宍懺曄壔偵偍偄偰偼丄婡擻暘巕孮偑惓偟偄帪偵惓偟偄応強偵塣偽傟傞昁梫偑偁傝偦偺桝憲偺惂屼婡峔丄偮傑傝偼嵶朎撪儘僕僗僥傿僋僗偺杮懱傪夝柧偡傞偙偲偼丄崅師婡擻僔僗僥儉偺攋抅偵婎偯偔幘姵偺昦懺傪峫偊傞忋偱傕晄壜寚偱偁傞丅巹嫟偺尋媶幒偱偼丄條乆側崅師婡擻僔僗僥儉偺婎斦偲側傞忋旂慻怐偺峔抸傗崅搙側恄宱婡擻偺婎斦偲側傞恄宱夞楬栐宍惉偵偍偗傞嵶朎撪儘僕僗僥傿僋僗偵拲栚偟丄偙傟傑偱偵Rab13掅暘巕検G抈敀幙偲偦偺昗揑抈敀幙JRAB偑丄忋旂嵶朎偺嵶朎娫愙拝傗恄宱嵶朎偺撍婲宍惉偵偍偄偰廳梫側栶妱傪扴偭偰偄傞壜擻惈傪採帵偟偰偒偨丅偙傟傜偺夁掱偱堷偒婲偙偝傟傞嵶朎崪奿偺嵞曇惉偼丄婡擻暘巕孮偺桝憲偲枾愙偵儕儞僋偟偰偍傝丄忋旂嵶朎偲恄宱嵶朎偵偍偄偰尒傜傟傞晛曊揑側儊僇僯僘儉偲峫偊傜傟傞丅崱夞偺戝妛堾DC僙儈僫乕偱偼丄偦偺儕儞僋婡峔偵偍偗傞Rab13-JRAB宯偺栶妱偲嶌梡婡峔偵偮偄偰丄偙傟傑偱偺惉壥傪偍榖偟偨偄丅 丂丂丂丂