尋媶撪梕
乮嶁杮乯嵶朎偺暘壔媦傃惗懚偵娭梌偡傞RNA戙幱婡峔偺夝柧丂偙傟傑偱偵丄擇杮嵔RNA寢崌抈敀幙(double-stranded RNA binding proteins:DRBPs)偼15庬椶埲忋偑尒弌偩偝傟偰偍傝丄揮幨丄東栿丄mRNA桝憲丄mRNA埨掕壔丄RNA曇廤丄microRNA(miRNA)惗崌惉摍偵娭梌偡傞偙偲偑曬崘偝傟偰偄傞 (Saunders et al. FASEB J 2003)丅DRBPs偼偦偺峔憿忋偵65-68巆婎埲忋偐傜側傞擇杮嵔RNA寢崌僪儊僀儞傪桳偟偰偍傝丄偙偺峔憿偲擇杮嵔偺妀巁偲偑寢崌偡傞偙偲偑抦傜傟偰偄傞丅DRBPs偼擇杮嵔妀巁偲寢崌偡傞偙偲偱丄寢崌偟偨妀巁偑娭梌偡傞忋婰偺嵶朎撪僀儀儞僩傪惂屼偟丄榁壔傗偑傫壔偲偄偭偨條乆側惗柦尰徾偵塭嬁傪媦傏偡偙偲偑曬崘偝傟偰偄傞 (Tominaga-Yamanaka et al. Aging 2012, Hu et al. Oncogene 2012)丅
丂DRBPs偺傂偲偮偱偁傞Nuclear Factor 90 (NF90)偼丄偦偺峔憿忋偵2偮偺擇杮嵔RNA寢崌僪儊僀儞傪桳偟丄庡偵妀偵嬊嵼偡傞偙偲偑暘偐偭偰偄傞丅 変乆偼偙傟傑偱偵丄夁忚敪尰偟偨NF90偲寢崌僷乕僩僫乕偱偁傞NF45偲偺暋崌懱 (NF90-NF45) 偑丄miRNA偺惗崌惉傪晧偵惂屼偡傞偙偲傪尒弌偟偨 (恾侾) (Sakamoto et al. MCB 2009)丅
丂miRNA偼21-24墫婎偐傜側傞彫暘巕偺旕東栿RNA偱丄憡曗揑丄堦晹憡曗揑側mRNA偺東栿傪梷惂偡傞丅偙偺婡擻傪捠偠偰丄miRNA偼嵶朎偺暘壔丄憹怋丄傾億僩乕僔僗摍偵娭梌偡傞偙偲偑暘偐偭偰偍傝丄敪惗傗柶塽挷愡摍偺惗懱惂屼偱廳梫側栶妱傪壥偨偡丅傑偨丄偑傫丄怱幘姵丄僂僀儖僗惈姶愼徢丄戙幱惈幘姵摍丄條乆側幘姵敪徢偲偺娭學傕尒弌偝傟偰偄傞丅
丂miRNA偺惗崌惉偵娭偟偰偼埲壓偺偙偲偑柧傜偐偲側偭偰偄傞丅miRNA偺弶婜揮幨嶻暔 (pri-miRNA) 偼Drosha/DGCR8偐傜側傞儅僀僋儘僾儘僙僢僒乕偵傛傝慜嬱懱miRNA (pre-miRNA) 傊僾儘僙僢僔儞僌偝傟傞丅pre-miRNA偼嵶朎幙偵桝憲偝傟偨屻丄偝傜偵僾儘僙僢僔儞僌傪庴偗丄惉弉宆miRNA偲側傞丅
丂変乆偼丄NF90-NF45偑pri-miRNA偵寢崌偡傞偲丄儅僀僋儘僾儘僙僢僒乕偑pri-miRNA傊愙嬤偱偒偢偵丄miRNA偺惗崌惉傪梷惂偡傞偙偲傪尒弌偟偨 (恾侾) (Sakamoto et al. MCB. 2009)丅
丂偙偺抦尒傪傕偲偵変乆偼丄NF90-NF45偼miRNA偺惗崌惉惂屼傪夘偟偰丄miRNA偑娭梌偡傞條乆側惗懱惂屼傗幘姵敪徢偵塭嬁傪媦傏偟偰偄傞偺偱偼側偄偐偲憐掕偟丄偦偺専徹傪恑傔偰偄傞丅
侾丏嵶朎庮釃壔偵娭梌偡傞怴偨側儅僀僋儘RNA嶻惗惂屼婡峔
俀丏儅僀僋儘RNA偺惗崌惉惂屼傪夘偟偨怴偨側嬝惉弉婡峔